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bacterial expression vector pgs21a  (GenScript corporation)

 
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    GenScript corporation bacterial expression vector pgs21a
    A. Schematic diagram of the strategy and process for high and authentical expression of rLOC387715 in E. coli. B. EcoR I + Xho I digestion of <t>pGS21a-LOC387715(ORF)m,</t> showing the expected size of insert (336 bp). C. SDS-PAGE, showing the high expression of the recombinant protein in bacterium BL21(DE3) strain after induction with IPTG (1mM at 37°C for 3 h). The recombinant protein rLOC387715 was about 15% of the bacterial total proteins. Top arrow, the recombinant protein – His-tagged GST-LOC387715 fusion protein that was expressed from pGS21a-LOC387715(ORF)m; bottom arrow, His-tagged GST that was expressed from the vector pGS21a. Std – protein standards.
    Bacterial Expression Vector Pgs21a, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bacterial expression vector pgs21a/product/GenScript corporation
    Average 90 stars, based on 1 article reviews
    bacterial expression vector pgs21a - by Bioz Stars, 2026-05
    90/100 stars

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    1) Product Images from "Expression of Recombinant Protein Encoded by LOC387715 in Escherichia coli"

    Article Title: Expression of Recombinant Protein Encoded by LOC387715 in Escherichia coli

    Journal:

    doi: 10.1016/j.pep.2007.03.017

    A. Schematic diagram of the strategy and process for high and authentical expression of rLOC387715 in E. coli. B. EcoR I + Xho I digestion of pGS21a-LOC387715(ORF)m, showing the expected size of insert (336 bp). C. SDS-PAGE, showing the high expression of the recombinant protein in bacterium BL21(DE3) strain after induction with IPTG (1mM at 37°C for 3 h). The recombinant protein rLOC387715 was about 15% of the bacterial total proteins. Top arrow, the recombinant protein – His-tagged GST-LOC387715 fusion protein that was expressed from pGS21a-LOC387715(ORF)m; bottom arrow, His-tagged GST that was expressed from the vector pGS21a. Std – protein standards.
    Figure Legend Snippet: A. Schematic diagram of the strategy and process for high and authentical expression of rLOC387715 in E. coli. B. EcoR I + Xho I digestion of pGS21a-LOC387715(ORF)m, showing the expected size of insert (336 bp). C. SDS-PAGE, showing the high expression of the recombinant protein in bacterium BL21(DE3) strain after induction with IPTG (1mM at 37°C for 3 h). The recombinant protein rLOC387715 was about 15% of the bacterial total proteins. Top arrow, the recombinant protein – His-tagged GST-LOC387715 fusion protein that was expressed from pGS21a-LOC387715(ORF)m; bottom arrow, His-tagged GST that was expressed from the vector pGS21a. Std – protein standards.

    Techniques Used: Expressing, SDS Page, Recombinant, Plasmid Preparation

    A. Detection by anti-GST antibody. B. Detection by anti-6xHis antibody. High expression of the recombinant protein was obtained in bacterium BL21(DE3) strain by the induction of IPTG (1 mM at 37°C for 3 h). Top arrow, the recombinant protein – His-tagged GST-LOC387715 fusion protein that was expressed from pGS21a-LOC387715(ORF)m; bottom arrow, His-tagged GST that was expressed from the vector pGS21a.
    Figure Legend Snippet: A. Detection by anti-GST antibody. B. Detection by anti-6xHis antibody. High expression of the recombinant protein was obtained in bacterium BL21(DE3) strain by the induction of IPTG (1 mM at 37°C for 3 h). Top arrow, the recombinant protein – His-tagged GST-LOC387715 fusion protein that was expressed from pGS21a-LOC387715(ORF)m; bottom arrow, His-tagged GST that was expressed from the vector pGS21a.

    Techniques Used: Expressing, Recombinant, Plasmid Preparation



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    bacterial expression vector pgs21a  (GenScript corporation)
    90
    GenScript corporation bacterial expression vector pgs21a
    A. Schematic diagram of the strategy and process for high and authentical expression of rLOC387715 in E. coli. B. EcoR I + Xho I digestion of <t>pGS21a-LOC387715(ORF)m,</t> showing the expected size of insert (336 bp). C. SDS-PAGE, showing the high expression of the recombinant protein in bacterium BL21(DE3) strain after induction with IPTG (1mM at 37°C for 3 h). The recombinant protein rLOC387715 was about 15% of the bacterial total proteins. Top arrow, the recombinant protein – His-tagged GST-LOC387715 fusion protein that was expressed from pGS21a-LOC387715(ORF)m; bottom arrow, His-tagged GST that was expressed from the vector pGS21a. Std – protein standards.
    Bacterial Expression Vector Pgs21a, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bacterial expression vector pgs21a/product/GenScript corporation
    Average 90 stars, based on 1 article reviews
    bacterial expression vector pgs21a - by Bioz Stars, 2026-05
    90/100 stars
    		  Buy from Supplier
    the bacterial expression vector pgs21a  (GenScript corporation)
    90
    GenScript corporation the bacterial expression vector pgs21a
    A. Schematic diagram of the strategy and process for high and authentical expression of rLOC387715 in E. coli. B. EcoR I + Xho I digestion of <t>pGS21a-LOC387715(ORF)m,</t> showing the expected size of insert (336 bp). C. SDS-PAGE, showing the high expression of the recombinant protein in bacterium BL21(DE3) strain after induction with IPTG (1mM at 37°C for 3 h). The recombinant protein rLOC387715 was about 15% of the bacterial total proteins. Top arrow, the recombinant protein – His-tagged GST-LOC387715 fusion protein that was expressed from pGS21a-LOC387715(ORF)m; bottom arrow, His-tagged GST that was expressed from the vector pGS21a. Std – protein standards.
    The Bacterial Expression Vector Pgs21a, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/the bacterial expression vector pgs21a/product/GenScript corporation
    Average 90 stars, based on 1 article reviews
    the bacterial expression vector pgs21a - by Bioz Stars, 2026-05
    90/100 stars
    		  Buy from Supplier

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    A. Schematic diagram of the strategy and process for high and authentical expression of rLOC387715 in E. coli. B. EcoR I + Xho I digestion of pGS21a-LOC387715(ORF)m, showing the expected size of insert (336 bp). C. SDS-PAGE, showing the high expression of the recombinant protein in bacterium BL21(DE3) strain after induction with IPTG (1mM at 37°C for 3 h). The recombinant protein rLOC387715 was about 15% of the bacterial total proteins. Top arrow, the recombinant protein – His-tagged GST-LOC387715 fusion protein that was expressed from pGS21a-LOC387715(ORF)m; bottom arrow, His-tagged GST that was expressed from the vector pGS21a. Std – protein standards.

    Journal:

    Article Title: Expression of Recombinant Protein Encoded by LOC387715 in Escherichia coli

    doi: 10.1016/j.pep.2007.03.017

    Figure Lengend Snippet: A. Schematic diagram of the strategy and process for high and authentical expression of rLOC387715 in E. coli. B. EcoR I + Xho I digestion of pGS21a-LOC387715(ORF)m, showing the expected size of insert (336 bp). C. SDS-PAGE, showing the high expression of the recombinant protein in bacterium BL21(DE3) strain after induction with IPTG (1mM at 37°C for 3 h). The recombinant protein rLOC387715 was about 15% of the bacterial total proteins. Top arrow, the recombinant protein – His-tagged GST-LOC387715 fusion protein that was expressed from pGS21a-LOC387715(ORF)m; bottom arrow, His-tagged GST that was expressed from the vector pGS21a. Std – protein standards.

    Article Snippet: LOC387715 cDNA synthesis, cloning and sequencing Self-designed synonymously modified ORF cDNA sequence of LOC387715 – LOC387715(ORF)m was synthesized and cloned in-frame into the bacterial expression vector pGS21a between EcoR I and Xho I sites by GenScript Corporation.

    Techniques: Expressing, SDS Page, Recombinant, Plasmid Preparation

    A. Detection by anti-GST antibody. B. Detection by anti-6xHis antibody. High expression of the recombinant protein was obtained in bacterium BL21(DE3) strain by the induction of IPTG (1 mM at 37°C for 3 h). Top arrow, the recombinant protein – His-tagged GST-LOC387715 fusion protein that was expressed from pGS21a-LOC387715(ORF)m; bottom arrow, His-tagged GST that was expressed from the vector pGS21a.

    Journal:

    Article Title: Expression of Recombinant Protein Encoded by LOC387715 in Escherichia coli

    doi: 10.1016/j.pep.2007.03.017

    Figure Lengend Snippet: A. Detection by anti-GST antibody. B. Detection by anti-6xHis antibody. High expression of the recombinant protein was obtained in bacterium BL21(DE3) strain by the induction of IPTG (1 mM at 37°C for 3 h). Top arrow, the recombinant protein – His-tagged GST-LOC387715 fusion protein that was expressed from pGS21a-LOC387715(ORF)m; bottom arrow, His-tagged GST that was expressed from the vector pGS21a.

    Article Snippet: LOC387715 cDNA synthesis, cloning and sequencing Self-designed synonymously modified ORF cDNA sequence of LOC387715 – LOC387715(ORF)m was synthesized and cloned in-frame into the bacterial expression vector pGS21a between EcoR I and Xho I sites by GenScript Corporation.

    Techniques: Expressing, Recombinant, Plasmid Preparation

    A. Schematic diagram of the strategy and process for high and authentical expression of rLOC387715 in E. coli. B. EcoR I + Xho I digestion of pGS21a-LOC387715(ORF)m, showing the expected size of insert (336 bp). C. SDS-PAGE, showing the high expression of the recombinant protein in bacterium BL21(DE3) strain after induction with IPTG (1mM at 37°C for 3 h). The recombinant protein rLOC387715 was about 15% of the bacterial total proteins. Top arrow, the recombinant protein – His-tagged GST-LOC387715 fusion protein that was expressed from pGS21a-LOC387715(ORF)m; bottom arrow, His-tagged GST that was expressed from the vector pGS21a. Std – protein standards.

    Journal:

    Article Title: Expression of Recombinant Protein Encoded by LOC387715 in Escherichia coli

    doi: 10.1016/j.pep.2007.03.017

    Figure Lengend Snippet: A. Schematic diagram of the strategy and process for high and authentical expression of rLOC387715 in E. coli. B. EcoR I + Xho I digestion of pGS21a-LOC387715(ORF)m, showing the expected size of insert (336 bp). C. SDS-PAGE, showing the high expression of the recombinant protein in bacterium BL21(DE3) strain after induction with IPTG (1mM at 37°C for 3 h). The recombinant protein rLOC387715 was about 15% of the bacterial total proteins. Top arrow, the recombinant protein – His-tagged GST-LOC387715 fusion protein that was expressed from pGS21a-LOC387715(ORF)m; bottom arrow, His-tagged GST that was expressed from the vector pGS21a. Std – protein standards.

    Article Snippet: Self-designed synonymously modified ORF cDNA sequence of LOC387715 – LOC387715(ORF)m was synthesized and cloned in-frame into the bacterial expression vector pGS21a between EcoR I and Xho I sites by GenScript Corporation.

    Techniques: Expressing, SDS Page, Recombinant, Plasmid Preparation

    A. Detection by anti-GST antibody. B. Detection by anti-6xHis antibody. High expression of the recombinant protein was obtained in bacterium BL21(DE3) strain by the induction of IPTG (1 mM at 37°C for 3 h). Top arrow, the recombinant protein – His-tagged GST-LOC387715 fusion protein that was expressed from pGS21a-LOC387715(ORF)m; bottom arrow, His-tagged GST that was expressed from the vector pGS21a.

    Journal:

    Article Title: Expression of Recombinant Protein Encoded by LOC387715 in Escherichia coli

    doi: 10.1016/j.pep.2007.03.017

    Figure Lengend Snippet: A. Detection by anti-GST antibody. B. Detection by anti-6xHis antibody. High expression of the recombinant protein was obtained in bacterium BL21(DE3) strain by the induction of IPTG (1 mM at 37°C for 3 h). Top arrow, the recombinant protein – His-tagged GST-LOC387715 fusion protein that was expressed from pGS21a-LOC387715(ORF)m; bottom arrow, His-tagged GST that was expressed from the vector pGS21a.

    Article Snippet: Self-designed synonymously modified ORF cDNA sequence of LOC387715 – LOC387715(ORF)m was synthesized and cloned in-frame into the bacterial expression vector pGS21a between EcoR I and Xho I sites by GenScript Corporation.

    Techniques: Expressing, Recombinant, Plasmid Preparation